CD1d-Restricted and TCR-Mediated Activation of Vα14 NKT Cells by Glycosylceramides

T Kawano, J Cui, Y Koezuka, I Toura, Y Kaneko… - Science, 1997 - science.org
T Kawano, J Cui, Y Koezuka, I Toura, Y Kaneko, K Motoki, H Ueno, R Nakagawa, H Sato…
Science, 1997science.org
Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR)
encoded by the Vα14 and Jα281 gene segments. A glycosylceramide-containing α-
anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was
identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of
Vα14 NKT cells were abrogated by treatment with chloroquine–concanamycin A or by
monoclonal antibodies against CD1d/Vβ8, CD40/CD40L, or B7/CTLA-4/CD28, but not by …
Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Vα14 and Jα281 gene segments. A glycosylceramide-containing α-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Vα14 NKT cells were abrogated by treatment with chloroquine–concanamycin A or by monoclonal antibodies against CD1d/Vβ8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.
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