Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes
K Tang, D Fu, S Kötter, RJ Cotter… - Nucleic acids …, 1995 - academic.oup.com
K Tang, D Fu, S Kötter, RJ Cotter, CR Cantor, H Köster
Nucleic acids research, 1995•academic.oup.comMatrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry
was used to analyze short DNA duplex probes with one strand immobilized on solid
supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-
Immobilized strand could be detected. Partial denaturation was found when the duplex
probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has
several applications, such as fast DNA sequence analysis and DNA diagnostics.
was used to analyze short DNA duplex probes with one strand immobilized on solid
supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-
Immobilized strand could be detected. Partial denaturation was found when the duplex
probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has
several applications, such as fast DNA sequence analysis and DNA diagnostics.
Abstract
Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.
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