Two‐dimensional (2‐D) maps of cytosol and enriched‐membrane platelet proteins has allowed the identification of more than 25 spots by three different methods: matching of the platelet gels with other 2‐D reference maps, immunoblotting with chemiluminescence detection, and N‐terminal sequencing. Different G protein (guanosine triphosphate‐binding protein) subunits, cytoskeletal proteins, and proteins common to the human liver, red blood cells and plasma were identified. The two platelet protein maps presented here contribute to the project of identification of human cell and body fluid proteins. They may serve as working tools since platelets are popular models for the study of central nervous system neurotransmitter systems and stimulus‐response coupling mechanisms.