[HTML][HTML] Amino acid residues in the αIIb subunit that are critical for ligand binding to integrin αIIbβ3 are clustered in the β-propeller model
T Kamata, KK Tieu, A Irie, TA Springer… - Journal of Biological …, 2001 - ASBMB
Several distinct regions of the integrin α IIb subunit have been implicated in ligand binding.
To localize the ligand binding sites in α IIb, we swapped all 27 predicted loops with the
corresponding sequences of α 4 or α 5. 19 of the 27 swapping mutations had no effect on
binding to both fibrinogen and ligand-mimetic antibodies (eg LJ-CP3), suggesting that these
regions do not contain major ligand binding sites. In contrast, swapping the remaining 8
predicted loops completely blocked ligand binding. Ala scanning mutagenesis of these …
To localize the ligand binding sites in α IIb, we swapped all 27 predicted loops with the
corresponding sequences of α 4 or α 5. 19 of the 27 swapping mutations had no effect on
binding to both fibrinogen and ligand-mimetic antibodies (eg LJ-CP3), suggesting that these
regions do not contain major ligand binding sites. In contrast, swapping the remaining 8
predicted loops completely blocked ligand binding. Ala scanning mutagenesis of these …