[HTML][HTML] Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848

M Jurk, F Heil, J Vollmer, C Schetter, AM Krieg… - Nature …, 2002 - nature.com
M Jurk, F Heil, J Vollmer, C Schetter, AM Krieg, H Wagner, G Lipford, S Bauer
Nature immunology, 2002nature.com
TLR8 or TLR7. HEK293 cells were transfected by electroporation with vectors expressing
human TLR8, human TLR7 or murine TLR7 and a NF-kB luciferase reporter plasmid.
Sixteen hours after transfection, cells were stimulated with R-848 (commercially synthesized
by GLSynthesis, Worcester, MA) at the indicated concentrations for a further 7 h. TLR2-,
TLR3-and TLR4-transfected cells did not induce NF-kB activation after stimulation with R-
848; a negative result was also scored for murine TLR8 (data not shown10). Data are …
TLR8 or TLR7. HEK293 cells were transfected by electroporation with vectors expressing human TLR8, human TLR7 or murine TLR7 and a NF-kB luciferase reporter plasmid. Sixteen hours after transfection, cells were stimulated with R-848 (commercially synthesized by GLSynthesis, Worcester, MA) at the indicated concentrations for a further 7 h. TLR2-, TLR3-and TLR4-transfected cells did not induce NF-kB activation after stimulation with R-848; a negative result was also scored for murine TLR8 (data not shown10). Data are meanąsd from one representative of three independent experiments.
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