Upregulation of intragraft interleukin-10 by infusion of granulocyte colony-stimulating factor-mobilized donor leukocytes

H Egi, K Hayamizu, I Ohmori, T Kitayama… - Transplant …, 2002 - Springer
H Egi, K Hayamizu, I Ohmori, T Kitayama, T Asahara
Transplant international, 2002Springer
We examined the effects of granulocyte colony-stimulating factor (G-CSF)-mobilized donor
leukocyte infusion (G-DLI) on facilitation of allograft survival using heart transplantation from
DA to Lewis rats that were transiently treated with tacrolimus (2 mg/kg im on day 0). Other
DA rats were given G-CSF (250 μg/kg/day sc from days–5 to 0), and isolated leukocytes
were infused into Lewis recipients after surgery. Cytokine mRNA levels were quantified by
reverse transcription and real-time polymerase chain reaction. After G-CSF treatment …
Abstract
We examined the effects of granulocyte colony-stimulating factor (G-CSF)-mobilized donor leukocyte infusion (G-DLI) on facilitation of allograft survival using heart transplantation from DA to Lewis rats that were transiently treated with tacrolimus (2 mg/kg i.m. on day 0). Other DA rats were given G-CSF (250 μg/kg/day s.c. from days –5 to 0), and isolated leukocytes were infused into Lewis recipients after surgery. Cytokine mRNA levels were quantified by reverse transcription and real-time polymerase chain reaction. After G-CSF treatment, leukocytes in circulation increased by 7.6 times and secreted in-vitro 6.0-times-higher levels of IL-10 after lipopolysaccharide stimulation than did untreated leukocytes. G-DLI facilitated graft survival dose-dependently. Significant IL-10 mRNA upregulation was detected in grafts 24 h after surgery but not in the recipient's heart, spleen, or liver. On day 6, IFN-γ and IL-2 mRNA levels were approximately half those of the control levels. Allograft-restricted IL-10 upregulation followed by type-1 cytokine downregulation can be achieved by the use of G-DLI.
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