Polymorphonuclear neutrophils promote rFGF-2-induced angiogenesis in vivo
JP Shaw, N Chuang, H Yee, P Shamamian - Journal of Surgical Research, 2003 - Elsevier
JP Shaw, N Chuang, H Yee, P Shamamian
Journal of Surgical Research, 2003•ElsevierBackground. The role of neutrophils in angiogenesis remains largely unknown. Recent
evidence has shown that polymorphonuclear neutrophils (PMNs) produce several
proangiogenic cytokines, including VEGF, TNF-α, IL-1, IL-6, and IL-8. In addition, PMN-
derived proteinases promote endothelial cell migration. We hypothesized that PMNs may
facilitate angiogenesis and that reducing circulating PMNs might alter the host angiogenic
response. Materials and methods. We utilized a corneal pocket assay to compare rFGF-2 …
evidence has shown that polymorphonuclear neutrophils (PMNs) produce several
proangiogenic cytokines, including VEGF, TNF-α, IL-1, IL-6, and IL-8. In addition, PMN-
derived proteinases promote endothelial cell migration. We hypothesized that PMNs may
facilitate angiogenesis and that reducing circulating PMNs might alter the host angiogenic
response. Materials and methods. We utilized a corneal pocket assay to compare rFGF-2 …
Background
The role of neutrophils in angiogenesis remains largely unknown. Recent evidence has shown that polymorphonuclear neutrophils (PMNs) produce several proangiogenic cytokines, including VEGF, TNF-α, IL-1, IL-6, and IL-8. In addition, PMN-derived proteinases promote endothelial cell migration. We hypothesized that PMNs may facilitate angiogenesis and that reducing circulating PMNs might alter the host angiogenic response.
Materials and methods
We utilized a corneal pocket assay to compare rFGF-2-induced vessel formation in the corneas of mice with normal levels of circulating neutrophils to those in a neutropenic state. Circulating PMNs were reduced using serial intraperitoneal injections of monoclonal antibody to Gr-1. Slow release rFGF2 pellets were implanted into the corneas of neutropenic mice and controls. Corneal neovascularization, measured as vessel length and area of vessel in-growth, was quantified using slit-lamp microscopy on day 7.
Results
The average number of circulating PMNs was significantly reduced in the experimental group compared to the control group on days 1–7 (P < 0.05). No statistical differences in circulating monocytes or lymphocytes were observed from days 0 to 6. Mice in the experimental group had a vascular area of 2.58 ± 0.2 mm2 compared to 3.55 ± 0.3 mm2 in the control group (P < 0.05).
Conclusions
Corneal neovascularization in response to rFGF-2 is diminished by PMN depletion. PMNs play an important role in facilitating rFGF-2-induced angiogenesis.
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