Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical application
B Thurner, C Röder, D Dieckmann, M Heuer… - Journal of …, 1999 - Elsevier
Dendritic Cell (DC)-based vaccination approaches in man require a reproducible DC
generation method that can be performed in conformity with GMP (Good Manufacturing
Practice) guidelines and that circumvents the need for multiple blood drawings to generate
DC. To this end we modified our previously described method to generate mature DC from
CD14+ monocytes by a two step method (priming in GM-SF+ IL-4 followed by maturation in
monocyte conditioned medium) for use with leukapheresis products as a starting population …
generation method that can be performed in conformity with GMP (Good Manufacturing
Practice) guidelines and that circumvents the need for multiple blood drawings to generate
DC. To this end we modified our previously described method to generate mature DC from
CD14+ monocytes by a two step method (priming in GM-SF+ IL-4 followed by maturation in
monocyte conditioned medium) for use with leukapheresis products as a starting population …