Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants
EKW Hui, PC Wang, SJ Lo - Cellular and Molecular Life Sciences CMLS, 1998 - Springer
EKW Hui, PC Wang, SJ Lo
Cellular and Molecular Life Sciences CMLS, 1998•SpringerMany virus and transposon DNAs can integrate into the host genome. In this review,
techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and
vectorette-or splinkerette-PCR are introduced as possible strategies for cloning flanking
DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture
PCR, and panhandle PCR and boomerang DNA amplification are also described. The
principles, advantages and limitations of each approach are discussed.
techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and
vectorette-or splinkerette-PCR are introduced as possible strategies for cloning flanking
DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture
PCR, and panhandle PCR and boomerang DNA amplification are also described. The
principles, advantages and limitations of each approach are discussed.
Abstract
Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.
Springer