Role of the autophagic‐lysosomal system on low potassium‐induced apoptosis in cultured cerebellar granule cells

N Canu, R Tufi, AL Serafino, G Amadoro… - Journal of …, 2005 - Wiley Online Library
N Canu, R Tufi, AL Serafino, G Amadoro, MT Ciotti, P Calissano
Journal of neurochemistry, 2005Wiley Online Library
Apoptotic and autophagic cell death have been implicated, on the basis of morphological
and biochemical criteria, in neuronal loss occurring in neurodegenerative diseases and it
has been shown that they may overlap. We have studied the relationship between apoptosis
and autophagic cell death in cerebellar granule cells (CGCs) undergoing apoptosis
following serum and potassium deprivation. We found that apoptosis is accompanied by an
early and marked proliferation of autophagosomal–lysosomal compartments as detected by …
Abstract
Apoptotic and autophagic cell death have been implicated, on the basis of morphological and biochemical criteria, in neuronal loss occurring in neurodegenerative diseases and it has been shown that they may overlap. We have studied the relationship between apoptosis and autophagic cell death in cerebellar granule cells (CGCs) undergoing apoptosis following serum and potassium deprivation. We found that apoptosis is accompanied by an early and marked proliferation of autophagosomal–lysosomal compartments as detected by electron microscopy and immunofluorescence analysis. Autophagy is blocked by hrIGF‐1 and forskolin, two well‐known inhibitors of CGC apoptosis, as well as by adenovirus‐mediated overexpression of Bcl‐2. 3‐Methyladenine (3‐MA) an inhibitor of autophagy, not only arrests this event but it also blocks apoptosis. The neuroprotective effect of 3‐MA is accompanied by block of cytochrome c (cyt c) release in the cytosol and by inhibition of caspase‐3 activation which, in turn, appears to be mediated by cathepsin B, as CA074‐Me, a selective inhibitor of this enzyme, fully blocks the processing of pro‐caspase‐3. Immunofluorescence analysis demonstratesd that cathepsin B, normally confined inside the lysosomal‐endosomal compartment, is released during apoptosis into the cytosol where this enzyme may act as an execution protease. Collectively, these observations indicate that autophagy precedes and is causally connected with the subsequent onset of programmed death.
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