[HTML][HTML] Identification of Omi/HtrA2 as a mitochondrial apoptotic serine protease that disrupts inhibitor of apoptosis protein-caspase interaction
R Hegde, SM Srinivasula, ZJ Zhang, R Wassell… - Journal of Biological …, 2002 - ASBMB
To identify human proteins that bind to the Smac and caspase-9 binding pocket on the
baculoviral inhibitor of apoptosis protein (IAP) repeat 3 (BIR3) domain of human XIAP, we
used BIR3 as an affinity reagent, followed by elution with the BIR3 binding peptide AVPIA,
microsequencing, and mass spectrometry. The mature serine protease Omi (also known as
HtrA2) was identified as a mitochondrial direct BIR3-binding protein and a caspase activator.
Like mature Smac (also known as Diablo), mature Omi contains a conserved IAP-binding …
baculoviral inhibitor of apoptosis protein (IAP) repeat 3 (BIR3) domain of human XIAP, we
used BIR3 as an affinity reagent, followed by elution with the BIR3 binding peptide AVPIA,
microsequencing, and mass spectrometry. The mature serine protease Omi (also known as
HtrA2) was identified as a mitochondrial direct BIR3-binding protein and a caspase activator.
Like mature Smac (also known as Diablo), mature Omi contains a conserved IAP-binding …