Isolation and analysis of components of CD95 (APO-1/Fas) death-inducing signaling complex
C Scaffidi, PH Krammer, ME Peter - Methods, 1999 - Elsevier
C Scaffidi, PH Krammer, ME Peter
Methods, 1999•ElsevierCD95 (APO-1/Fas) is an apoptosis-inducing receptor belonging to the tumor necrosis factor
receptor superfamily. Multimerization of CD95 leads to instant recruitment of the signaling
molecules FADD and caspase-8 to the activated receptor forming the death-inducing
signaling complex (DISC). DISC formation is the first essential step of CD95 signaling and
results in activation of caspase-8 starting a signaling cascade that leads to apoptosis. Here
we describe a method for analyzing the CD95 DISC. The method is based on …
receptor superfamily. Multimerization of CD95 leads to instant recruitment of the signaling
molecules FADD and caspase-8 to the activated receptor forming the death-inducing
signaling complex (DISC). DISC formation is the first essential step of CD95 signaling and
results in activation of caspase-8 starting a signaling cascade that leads to apoptosis. Here
we describe a method for analyzing the CD95 DISC. The method is based on …
CD95 (APO-1/Fas) is an apoptosis-inducing receptor belonging to the tumor necrosis factor receptor superfamily. Multimerization of CD95 leads to instant recruitment of the signaling molecules FADD and caspase-8 to the activated receptor forming the death-inducing signaling complex (DISC). DISC formation is the first essential step of CD95 signaling and results in activation of caspase-8 starting a signaling cascade that leads to apoptosis. Here we describe a method for analyzing the CD95 DISC. The method is based on coimmunoprecipitation of the signaling molecules with the activated CD95 receptor followed by Western blot detection of associated molecules. Therefore, this method can analyze the very first signaling events during CD95-mediated apoptosis.
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