[PDF][PDF] Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast
Q Xu, JC Reed - Molecular cell, 1998 - cell.com
Q Xu, JC Reed
Molecular cell, 1998•cell.comThe mammalian proapoptotic protein Bax confers a lethal phenotype when expressed in
yeast. By exploiting this phenotype, we have identified a novel human Bax inhibitor, BI-1. BI-
1 is an evolutionarily conserved integral membrane protein containing multiple membrane-
spanning segments and is predominantly localized to intracellular membranes, similar to Bcl-
2 family proteins. Moreover, BI-1 can interact with Bcl-2 and Bcl-X L but not Bax or Bak, as
demonstrated by in vivo cross-linking and coimmunoprecipitation studies. When …
yeast. By exploiting this phenotype, we have identified a novel human Bax inhibitor, BI-1. BI-
1 is an evolutionarily conserved integral membrane protein containing multiple membrane-
spanning segments and is predominantly localized to intracellular membranes, similar to Bcl-
2 family proteins. Moreover, BI-1 can interact with Bcl-2 and Bcl-X L but not Bax or Bak, as
demonstrated by in vivo cross-linking and coimmunoprecipitation studies. When …
Abstract
The mammalian proapoptotic protein Bax confers a lethal phenotype when expressed in yeast. By exploiting this phenotype, we have identified a novel human Bax inhibitor, BI-1. BI-1 is an evolutionarily conserved integral membrane protein containing multiple membrane-spanning segments and is predominantly localized to intracellular membranes, similar to Bcl-2 family proteins. Moreover, BI-1 can interact with Bcl-2 and Bcl-XL but not Bax or Bak, as demonstrated by in vivo cross-linking and coimmunoprecipitation studies. When overexpressed in mammalian cells, BI-1 suppressed apoptosis induced by Bax, etoposide, staurosporine, and growth factor deprivation, but not by Fas (CD95). Conversely, BI-1 antisense induced apoptosis. BI-1 thus represents a new type of regulator of cell death pathways controlled by Bcl-2 and Bax.
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