Through suppressive subtractive hybridization, we identified a new gene whose
transcription is induced by sterol regulatory element-binding proteins (SREBPs). The gene
encodes acetyl-CoA synthetase (ACS), the cytosolic enzyme that activates acetate so that it
can be used for lipid synthesis or for energy generation. ACS genes were isolated
previously from yeast, but not from animal cells. Recombinant human ACS was produced by
expressing the cloned cDNA transiently in human cells. After purification by nickel …