[HTML][HTML] Isolation of sterol-resistant Chinese hamster ovary cells with genetic deficiencies in both Insig-1 and Insig-2
Insig-1 and Insig-2, a pair of endoplasmic reticulum (ER) membrane proteins, mediate
feedback control of cholesterol synthesis through their sterol-dependent binding to the
following two polytopic ER membrane proteins: sterol regulatory element-binding protein
(SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A
reductase. Sterol-induced binding of Insigs to SCAP prevents the proteolytic processing of
SREBPs, membrane-bound transcription factors that enhance the synthesis of cholesterol …
feedback control of cholesterol synthesis through their sterol-dependent binding to the
following two polytopic ER membrane proteins: sterol regulatory element-binding protein
(SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A
reductase. Sterol-induced binding of Insigs to SCAP prevents the proteolytic processing of
SREBPs, membrane-bound transcription factors that enhance the synthesis of cholesterol …