Multivalent control of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Mevalonate-derived product inhibits translation of mRNA and accelerates degradation of …
M Nakanishi, JL Goldstein, MS Brown - Journal of Biological Chemistry, 1988 - ASBMB
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) is
subject to multivalent feedback suppression mediated by sterols and non-sterol substances
derived from mevalonate, the product of the enzyme. To dissect the mechanism for this
multivalent effect, Chinese hamster ovary cells were incubated with sterols contained in
plasma lipoproteins and with a high concentration (100 microM) of compactin, an inhibitor of
the reductase. Under these conditions, the amounts of HMG-CoA reductase protein and …
subject to multivalent feedback suppression mediated by sterols and non-sterol substances
derived from mevalonate, the product of the enzyme. To dissect the mechanism for this
multivalent effect, Chinese hamster ovary cells were incubated with sterols contained in
plasma lipoproteins and with a high concentration (100 microM) of compactin, an inhibitor of
the reductase. Under these conditions, the amounts of HMG-CoA reductase protein and …