[HTML][HTML] G protein-independent activation of an inward Na+ current by muscarinic receptors in mouse pancreatic β-cells
JF Rolland, JC Henquin, P Gilon - Journal of biological chemistry, 2002 - ASBMB
Depolarization of pancreatic β-cells is critical for stimulation of insulin secretion by
acetylcholine but remains unexplained. Using voltage-clamped β-cells, we identified a small
inward current produced by acetylcholine, which was suppressed by atropine or external
Na+ omission, but was not mimicked by nicotine, and was insensitive to nicotinic
antagonists, tetrodotoxin, 4, 4′-diisothiocyanostilbene-2, 2′-disulfonic acid (DiDS),
thapsigargin pretreatment, and external Ca 2+ and K+ removal. This suggests that …
acetylcholine but remains unexplained. Using voltage-clamped β-cells, we identified a small
inward current produced by acetylcholine, which was suppressed by atropine or external
Na+ omission, but was not mimicked by nicotine, and was insensitive to nicotinic
antagonists, tetrodotoxin, 4, 4′-diisothiocyanostilbene-2, 2′-disulfonic acid (DiDS),
thapsigargin pretreatment, and external Ca 2+ and K+ removal. This suggests that …