[HTML][HTML] Direct photoaffinity labeling of the Kir6. 2 subunit of the ATP-sensitive K+ channel by 8-azido-ATP
K Tanabe, SJ Tucker, M Matsuo, P Proks… - Journal of Biological …, 1999 - ASBMB
ATP-sensitive potassium channels are under complex regulation by intracellular ATP and
ADP. The potentiating effect of MgADP is conferred by the sulfonylurea receptor subunit of
the channel, SUR, whereas the inhibitory effect of ATP appears to be mediated via the pore-
forming subunit, Kir6. 2. We determined whether ATP directly interacts with a binding site on
the Kir6. 2 subunit to mediate channel inhibition by analyzing binding of a photoaffinity
analog of ATP (8-azido-[γ-32 P] ATP) to membranes from COS-7 cells transiently expressing …
ADP. The potentiating effect of MgADP is conferred by the sulfonylurea receptor subunit of
the channel, SUR, whereas the inhibitory effect of ATP appears to be mediated via the pore-
forming subunit, Kir6. 2. We determined whether ATP directly interacts with a binding site on
the Kir6. 2 subunit to mediate channel inhibition by analyzing binding of a photoaffinity
analog of ATP (8-azido-[γ-32 P] ATP) to membranes from COS-7 cells transiently expressing …