[HTML][HTML] ClC-3 chloride channels facilitate endosomal acidification and chloride accumulation
M Hara-Chikuma, B Yang, ND Sonawane… - Journal of Biological …, 2005 - ASBMB
We investigated the involvement of ClC-3 chloride channels in endosomal acidification by
measurement of endosomal pH and chloride concentration [Cl–] in control versus ClC-3-
deficient hepatocytes and in control versus ClC-3-transfected Chinese hamster ovary cells.
Endosomes were labeled with pH or [Cl–]-sensing fluorescent transferrin (Tf), which targets
to early/recycling endosomes, or α 2-macroglobulin (α 2 M), which targets to late
endosomes. In pulse label-chase experiments,[Cl–] was 19 mm just after internalization in α …
measurement of endosomal pH and chloride concentration [Cl–] in control versus ClC-3-
deficient hepatocytes and in control versus ClC-3-transfected Chinese hamster ovary cells.
Endosomes were labeled with pH or [Cl–]-sensing fluorescent transferrin (Tf), which targets
to early/recycling endosomes, or α 2-macroglobulin (α 2 M), which targets to late
endosomes. In pulse label-chase experiments,[Cl–] was 19 mm just after internalization in α …