[HTML][HTML] Cloning and Functional Expression of Rat CLC-5, a Chloride Channel Related to Kidney Disease (∗)
K Steinmeyer, B Schwappach, M Bens… - Journal of Biological …, 1995 - ASBMB
We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5.
The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4,
with which it shares∼ 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel
anion currents. They are strongly outwardly rectifying and have a conductivity sequence of
NO 3−> Cl−> Br−> I−≫ glutamate−. Although CLC-5 has consensus sites for
phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no …
The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4,
with which it shares∼ 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel
anion currents. They are strongly outwardly rectifying and have a conductivity sequence of
NO 3−> Cl−> Br−> I−≫ glutamate−. Although CLC-5 has consensus sites for
phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no …