Degranulation from human eosinophils stimulated with C3a and C5a
K Tadokoro, CA Dahindenb - Int Arch Allergy Immunol, 1994 - karger.com
K Tadokoro, CA Dahindenb
Int Arch Allergy Immunol, 1994•karger.comIt is unclear what actually induces eosinophil degranulation in vivo. We exam ined
eosinophil cationic protein (ECP) release from normal human eosino phils (Eos) in response
to C3a and C5a. C3a and C5a induced remarkable ECP release when Eos were
preincubated with cytochalasin B. ECP release induced by C3a or C5a was greater than that
induced by PAF at a concentration of 10-7 M. The ED50 for ECP release was 3 x 10-8 M and
3 x 10-9 M for C3a and C5a, respectively. C3a or C5a elicited a rapid and transient rise in …
eosinophil cationic protein (ECP) release from normal human eosino phils (Eos) in response
to C3a and C5a. C3a and C5a induced remarkable ECP release when Eos were
preincubated with cytochalasin B. ECP release induced by C3a or C5a was greater than that
induced by PAF at a concentration of 10-7 M. The ED50 for ECP release was 3 x 10-8 M and
3 x 10-9 M for C3a and C5a, respectively. C3a or C5a elicited a rapid and transient rise in …
Abstract
It is unclear what actually induces eosinophil degranulation in vivo. We exam ined eosinophil cationic protein (ECP) release from normal human eosino phils (Eos) in response to C3a and C5a. C3a and C5a induced remarkable ECP release when Eos were preincubated with cytochalasin B. ECP release induced by C3a or C5a was greater than that induced by PAF at a concentration of 10-7 M. The ED50 for ECP release was 3 x 10-8 M and 3 x 10-9 M for C3a and C5a, respectively. C3a or C5a elicited a rapid and transient rise in [Ca2+] i. These results suggest that C3a and C5a may contribute to hypersensitivity diseases by inducing eosinophil degranulation.
Karger