[HTML][HTML] Transcriptionally active polymerase chain reaction (TAP): high throughput gene expression using genome sequence data
X Liang, A Teng, DM Braun, J Felgner, Y Wang… - Journal of Biological …, 2002 - ASBMB
An approach is described for makingtranscriptionally active PCR (TAP) fragments that were
used directly in in vitro and in vivo expression experiments. TAP fragments encoding
reporter genes were amplified in 1 day using typical PCR methodology and were expressed
in cultured cells and in mice at levels comparable with a widely used cytomegalovirus
promoter-based plasmid expression vector. Following intramuscular injection, a TAP
fragment encoding hepatitis B surface antigen (HBsAg) induced anti-HBsAg antibody titers …
used directly in in vitro and in vivo expression experiments. TAP fragments encoding
reporter genes were amplified in 1 day using typical PCR methodology and were expressed
in cultured cells and in mice at levels comparable with a widely used cytomegalovirus
promoter-based plasmid expression vector. Following intramuscular injection, a TAP
fragment encoding hepatitis B surface antigen (HBsAg) induced anti-HBsAg antibody titers …