[HTML][HTML] Dog mast cell α-chymase activates progelatinase B by cleaving the Phe88-Gln89 and Phe91-Glu92 bonds of the catalytic domain
KC Fang, WW Raymond, JL Blount… - Journal of Biological …, 1997 - ASBMB
In prior work we showed that a metallogelatinase is secreted from dog mastocytoma cells
and directly activated by exocytosed mast cell α-chymase. The current work identifies the
protease as a canine homologue of progelatinase B (92-kDa gelatinase, MMP-9),
determines the sites cleaved by α-chymase, and explores the regulation of gelatinase
expression in mastocytoma cells. To obtain a cDNA encoding the complete sequence of
mastocytoma gelatinase B, a 2.3-kilobase clone encoding progelatinase was isolated from a …
and directly activated by exocytosed mast cell α-chymase. The current work identifies the
protease as a canine homologue of progelatinase B (92-kDa gelatinase, MMP-9),
determines the sites cleaved by α-chymase, and explores the regulation of gelatinase
expression in mastocytoma cells. To obtain a cDNA encoding the complete sequence of
mastocytoma gelatinase B, a 2.3-kilobase clone encoding progelatinase was isolated from a …