Enhanced association of plasminogen/plasmin with lesional epidermis of bullous pemphigoid

HM Gissler, MM Simon… - British Journal of …, 1992 - Wiley Online Library
HM Gissler, MM Simon, MD Kramer
British Journal of Dermatology, 1992Wiley Online Library
The distribution of plasminogen/plasmin, the central proteolytic component of the
plasminogen activator/plasmin system was analysed in lesional skin of bullous pemphigoid
by using monoclonal antibodies (MAbs) specific for distinct epitopes of the
plasminogen/plasmin molecule. Four groups of MAbs were used:(i) MAbs HD‐PG 1 and HD‐
PG 2. specific for epitopes associated with the lysinebinding sites I (kringle domain 1+ 2+ 3)
and II (kringle domain 4) of plasminogen/plasmin,(ii) MAbs HD‐PG 6 and HD‐PG 7, specific …
Summary
The distribution of plasminogen/plasmin, the central proteolytic component of the plasminogen activator/plasmin system was analysed in lesional skin of bullous pemphigoid by using monoclonal antibodies (MAbs) specific for distinct epitopes of the plasminogen/plasmin molecule. Four groups of MAbs were used: (i) MAbs HD‐PG 1 and HD‐PG 2. specific for epitopes associated with the lysinebinding sites I (kringle domain 1+ 2+3) and II (kringle domain 4) of plasminogen/plasmin, (ii) MAbs HD‐PG 6 and HD‐PG 7, specific for the lysine binding site I only, (iii) MAbs HD‐PG 12 (formerly designated P 2) and HD‐PG 18, specific for non‐kringle domains of glu‐and lys‐plasminogen, and (iv) MAb HD‐PG 13 which recognizes glu‐plasminogen. only The basal cell layers of normal skin consistently reacted with MAb HD‐PG 12, whereas only faint staining was seen with the other MAbs in the same biopsies. In contrast, all anti‐plasminogen/plasmin MAbs strongly stained lower and intermediate epidermal cell layers of fully developed bullous pemphigoid lesions.
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