Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa
IM Leigh, RAJ Eady, AHM Heagerty, PE Purkis… - Journal of investigative …, 1988 - Elsevier
IM Leigh, RAJ Eady, AHM Heagerty, PE Purkis, PA Whitehead, RE Burgeson
Journal of investigative dermatology, 1988•ElsevierThe murine monoclonal antibody LH 7: 2, which reacts with the basement membrane of
stratified squamous epithelia including epidermis, has been characterized biochemically
and shown to bind to part of the type VII collagen molecule. Immunoblotting reveals that the
antibody binding site lies in the non-helical carboxy terminal region of the type VII collagen
dimer and immunoelectron microscopy shows that the epitope is within the lamina densa of
the basement membrane. Loss of LH 7: 2 binding in the hereditary blistering disease …
stratified squamous epithelia including epidermis, has been characterized biochemically
and shown to bind to part of the type VII collagen molecule. Immunoblotting reveals that the
antibody binding site lies in the non-helical carboxy terminal region of the type VII collagen
dimer and immunoelectron microscopy shows that the epitope is within the lamina densa of
the basement membrane. Loss of LH 7: 2 binding in the hereditary blistering disease …
The murine monoclonal antibody LH 7:2, which reacts with the basement membrane of stratified squamous epithelia including epidermis, has been characterized biochemically and shown to bind to part of the type VII collagen molecule.
Immunoblotting reveals that the antibody binding site lies in the non-helical carboxy terminal region of the type VII collagen dimer and immunoelectron microscopy shows that the epitope is within the lamina densa of the basement membrane. Loss of LH 7:2 binding in the hereditary blistering disease recessive dystrophic epidermolysis bullosa suggests that inadequate synthesis or excessive breakdown of type VII collagen may form the biologic basis for the disease.
Elsevier