[HTML][HTML] Identification of RET autophosphorylation sites by mass spectrometry
Y Kawamoto, K Takeda, Y Okuno, Y Yamakawa… - Journal of Biological …, 2004 - ASBMB
The catalytic and signaling activities of RET, a receptor-type tyrosine kinase, are regulated
by the autophosphorylation of several tyrosine residues in the cytoplasmic region of RET.
Some studies have revealed a few possible autophosphorylation sites of RET by [32 P]
phosphopeptide mapping or by using specific anti-phosphotyrosine antibodies. To ultimately
identify these and other autophosphorylation sites of RET, we performed mass spectrometry
analysis of an originally prepared RET recombinant protein. Both the autophosphorylation …
by the autophosphorylation of several tyrosine residues in the cytoplasmic region of RET.
Some studies have revealed a few possible autophosphorylation sites of RET by [32 P]
phosphopeptide mapping or by using specific anti-phosphotyrosine antibodies. To ultimately
identify these and other autophosphorylation sites of RET, we performed mass spectrometry
analysis of an originally prepared RET recombinant protein. Both the autophosphorylation …