[HTML][HTML] Regulation of leucine-stimulated insulin secretion and glutamine metabolism in isolated rat islets
C Li, H Najafi, Y Daikhin, IB Nissim, HW Collins… - Journal of Biological …, 2003 - ASBMB
Glutamate dehydrogenase (GDH) is regulated by both positive (leucine and ADP) and
negative (GTP and ATP) allosteric factors. We hypothesized that the phosphate potential of β-
cells regulates the sensitivity of leucine stimulation. These predictions were tested by
measuring leucine-stimulated insulin secretion in perifused rat islets following glucose
depletion and by tracing the nitrogen flux of [2-15 N] glutamine using stable isotope
techniques. The sensitivity of leucine stimulation was enhanced by long time (120-min) …
negative (GTP and ATP) allosteric factors. We hypothesized that the phosphate potential of β-
cells regulates the sensitivity of leucine stimulation. These predictions were tested by
measuring leucine-stimulated insulin secretion in perifused rat islets following glucose
depletion and by tracing the nitrogen flux of [2-15 N] glutamine using stable isotope
techniques. The sensitivity of leucine stimulation was enhanced by long time (120-min) …