Isolation and characterization of a novel cDNA from HL-60 cells treated with 1, 25-dihydroxyvitamin D-3
KS Chen, HF DeLuca - Biochimica et Biophysica Acta (BBA)-Gene …, 1994 - Elsevier
A novel cDNA clone (VDUP1) has been isolated from a cDNA library constructed from
mRNA obtained from HL-60 cells stimulated by 1, 25-dihydroxyvitamin D-3. Northern blot
analysis showed that VDUP1 cDNA hybridizes to a 2.9 kb mRNA species which is up-
regulated in HL-60 cells by 1, 25-dihydroxyvitamin D-3 (1, 25-(OH) 2 D 3) treatment. In vitro
expression of VDUP1 cDNA produced a 46 kDa protein. A search of the GenBank database
revealed that the 3′ untranslated region of VDUP1 is homologous to a sequence …
mRNA obtained from HL-60 cells stimulated by 1, 25-dihydroxyvitamin D-3. Northern blot
analysis showed that VDUP1 cDNA hybridizes to a 2.9 kb mRNA species which is up-
regulated in HL-60 cells by 1, 25-dihydroxyvitamin D-3 (1, 25-(OH) 2 D 3) treatment. In vitro
expression of VDUP1 cDNA produced a 46 kDa protein. A search of the GenBank database
revealed that the 3′ untranslated region of VDUP1 is homologous to a sequence …