Bone marrow transplantation abolishes inhibition of arteriogenesis in placenta growth factor (PlGF)–/–mice
D Scholz, H Elsaesser, A Sauer, C Friedrich… - Journal of Molecular and …, 2003 - Elsevier
Journal of Molecular and Cellular Cardiology, 2003•Elsevier
We studied the influence of placenta growth factor (PlGF) on arteriogenesis and tested the
role of bone marrow (BM)-derived cells on PlGF-depleted vascular growth. Methods.–Right
femoral artery was occluded in wild type (+/+), PlGF ko (–/–) and (–/–) mice receiving BM
from (+/+) mice (–/–BM). Blood supply in paws was assessed by laser-Doppler imaging (LDI)
measurements before, immediately after, as well as 3, 7, 14, 21 and 28 days after surgery.
Seven and 28 days after occlusion, hindlimbs were perfusion fixed and filled with contrast …
role of bone marrow (BM)-derived cells on PlGF-depleted vascular growth. Methods.–Right
femoral artery was occluded in wild type (+/+), PlGF ko (–/–) and (–/–) mice receiving BM
from (+/+) mice (–/–BM). Blood supply in paws was assessed by laser-Doppler imaging (LDI)
measurements before, immediately after, as well as 3, 7, 14, 21 and 28 days after surgery.
Seven and 28 days after occlusion, hindlimbs were perfusion fixed and filled with contrast …
We studied the influence of placenta growth factor (PlGF) on arteriogenesis and tested the role of bone marrow (BM)-derived cells on PlGF-depleted vascular growth.
Methods
Right femoral artery was occluded in wild type (+/+), PlGF k.o. (–/–) and (–/–) mice receiving BM from (+/+) mice (–/– BM). Blood supply in paws was assessed by laser-Doppler imaging (LDI) measurements before, immediately after, as well as 3, 7, 14, 21 and 28 days after surgery. Seven and 28 days after occlusion, hindlimbs were perfusion fixed and filled with contrast medium. Angiograms were evaluated, collateral arteries were analysed histologically and morphometrically.
Results
Seven days after occlusion the blood flow and collateral growth in (–/–) were delayed in comparison to the (+/+) group. BM transplantation from +/+ donors prevented this delay. After 3 weeks the (–/–) mice reached values similar to the +/+ control.
Conclusion
PlGF is an important promoter of arteriogenesis. BM transplantation abolishes inhibition of arteriogenesis in PlGF –/– mice.
Elsevier