[HTML][HTML] Superoxide generation from endothelial nitric-oxide synthase: a Ca2+/calmodulin-dependent and tetrahydrobiopterin regulatory process
Y Xia, AL Tsai, V Berka, JL Zweier - Journal of Biological Chemistry, 1998 - ASBMB
It has been previously shown that besides synthesizing nitric oxide (NO), neuronal and
inducible NO synthase (NOS) generates superoxide (O⨪ 2) under conditions ofl-arginine
depletion. However, there is controversy regarding whether endothelial NOS (eNOS) can
also produce O⨪ 2. Moreover, the mechanism and control of this process are not fully
understood. Therefore, we performed electron paramagnetic resonance spin-trapping
experiments to directly measure and characterize the O⨪ 2 generation from purified eNOS …
inducible NO synthase (NOS) generates superoxide (O⨪ 2) under conditions ofl-arginine
depletion. However, there is controversy regarding whether endothelial NOS (eNOS) can
also produce O⨪ 2. Moreover, the mechanism and control of this process are not fully
understood. Therefore, we performed electron paramagnetic resonance spin-trapping
experiments to directly measure and characterize the O⨪ 2 generation from purified eNOS …