[HTML][HTML] Binding of serum response factor to CArG box sequences is necessary but not sufficient to restrict gene expression to arterial smooth muscle cells
M Strobeck, S Kim, JCL Zhang, C Clendenin… - Journal of Biological …, 2001 - ASBMB
Serum response factor (SRF) plays an important role in regulating smooth muscle cell
(SMC) development and differentiation. To understand the molecular mechanisms
underlying the activity of SRF in SMCs, the two CArG box-containing elements in the arterial
SMC-specific SM22α promoter, SME-1 and SME-4, were functionally and biochemically
characterized. Mutations that abolish binding of SRF to the SM22α promoter totally abolish
promoter activity in transgenic mice. Moreover, a multimerized copy of either SME-1 or SME …
(SMC) development and differentiation. To understand the molecular mechanisms
underlying the activity of SRF in SMCs, the two CArG box-containing elements in the arterial
SMC-specific SM22α promoter, SME-1 and SME-4, were functionally and biochemically
characterized. Mutations that abolish binding of SRF to the SM22α promoter totally abolish
promoter activity in transgenic mice. Moreover, a multimerized copy of either SME-1 or SME …