[HTML][HTML] Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the golgi apparatus
M Takahashi, H Shibata, M Shimakawa… - Journal of Biological …, 1999 - ASBMB
A novel 450-kDa coiled-coil protein, CG-NAP (centrosome and Golgi localized PKN-
associated protein), was identified as a protein that interacted with the regulatory region of
the protein kinase PKN, having a catalytic domain homologous to that of protein kinase C.
CG-NAP contains two sets of putative RII (regulatory subunit of protein kinase A)-binding
motif. Indeed, CG-NAP tightly bound to RIIα in HeLa cells. Furthermore, CG-NAP was
coimmunoprecipitated with the catalytic subunit of protein phosphatase 2A (PP2A), when …
associated protein), was identified as a protein that interacted with the regulatory region of
the protein kinase PKN, having a catalytic domain homologous to that of protein kinase C.
CG-NAP contains two sets of putative RII (regulatory subunit of protein kinase A)-binding
motif. Indeed, CG-NAP tightly bound to RIIα in HeLa cells. Furthermore, CG-NAP was
coimmunoprecipitated with the catalytic subunit of protein phosphatase 2A (PP2A), when …