We have developed an ultrasensitive isoelectrofocusing (IEF) method for the detection of
oligoclonal (OGC) IgG bands in cerebrospinal fluid (CSF) and sera. In this procedure,
double antibody and peroxidase immunodetection have been substituted by a single
antibody labelled with alkaline phosphatase. Alkaline phosphatase immunodetection not
only improves tenfold the sensitivity of the assay but also gives a sharper pattern resolution
making the interpretation easier and increases the specificity of this technique. This …