[HTML][HTML] De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxylase
JB Cheng, DL Motola, DJ Mangelsdorf… - Journal of Biological …, 2003 - ASBMB
The conversion of vitamin D into an active ligand for the vitamin D receptor requires 25-
hydroxylation in the liver and 1α-hydroxylation in the kidney. Mitochondrial and microsomal
vitamin D 25-hydroxylase enzymes catalyze the first reaction. The mitochondrial activity is
associated with sterol 27-hydroxylase, a cytochrome P450 (CYP27A1); however, the identity
of the microsomal enzyme has remained elusive. A cDNA library prepared from hepatic
mRNA of sterol 27-hydroxylase-deficient mice was screened with a ligand activation assay …
hydroxylation in the liver and 1α-hydroxylation in the kidney. Mitochondrial and microsomal
vitamin D 25-hydroxylase enzymes catalyze the first reaction. The mitochondrial activity is
associated with sterol 27-hydroxylase, a cytochrome P450 (CYP27A1); however, the identity
of the microsomal enzyme has remained elusive. A cDNA library prepared from hepatic
mRNA of sterol 27-hydroxylase-deficient mice was screened with a ligand activation assay …