[HTML][HTML] COOH-terminal disruption of lipoprotein lipase in mice is lethal in homozygotes, but heterozygotes have elevated triglycerides and impaired enzyme activity

T Coleman, RL Seip, JM Gimble, D Lee… - Journal of Biological …, 1995 - ASBMB
The role of the enzyme lipoprotein lipase (LPL) in atherosclerosis is uncertain. To generate
an animal model of LPL deficiency, we targeted the LPL gene in embryonic stem cells with a
vector designed to disrupt the COOH terminus of the protein and used these cells to
generate LPL-deficient mice. Germ line transmission of the disrupted LPL allele was
achieved with two chimeric males, and offspring from each of these animals were
phenotypically identical. Pups homozygous (-/-) for LPL deficiency died within 48 h of birth …