Advances in quantitative PCR technology: 5′ nuclease assays
YS Lie, CJ Petropoulos - Current opinion in biotechnology, 1998 - Elsevier
YS Lie, CJ Petropoulos
Current opinion in biotechnology, 1998•ElsevierThe development of 5′ nuclease assays represents a significant advance in nucleic acid
quantitation. This approach utilizes the 5′-3′ exonuclease activity of Thermus aquaticus
(Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during
amplification. The release of a fluorogenic tag from the 5′ end of the probe is proportional
to the target sequence concentration (copy number), and can be measured either at
endpoint (post-amplification), or in 'real time', where the increase in emission intensity is …
quantitation. This approach utilizes the 5′-3′ exonuclease activity of Thermus aquaticus
(Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during
amplification. The release of a fluorogenic tag from the 5′ end of the probe is proportional
to the target sequence concentration (copy number), and can be measured either at
endpoint (post-amplification), or in 'real time', where the increase in emission intensity is …
The development of 5′ nuclease assays represents a significant advance in nucleic acid quantitation. This approach utilizes the 5′-3′ exonuclease activity of Thermus aquaticus (Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during amplification. The release of a fluorogenic tag from the 5′ end of the probe is proportional to the target sequence concentration (copy number), and can be measured either at endpoint (post-amplification), or in ‘real time’, where the increase in emission intensity is followed on a per-cycle basis.
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