[HTML][HTML] Cloning of a unique lipase from endothelial cells extends the lipase gene family

K Hirata, HL Dichek, JA Cioffi, SY Choi… - Journal of Biological …, 1999 - ASBMB
A new lipoprotein lipase-like gene has been cloned from endothelial cells through a
subtraction methodology aimed at characterizing genes that are expressed with in
vitrodifferentiation of this cell type. The conceptual endothelial cell-derived lipase protein
contains 500 amino acids, including an 18-amino acid hydrophobic signal sequence, and is
44% identical to lipoprotein lipase and 41% identical to hepatic lipase. Comparison of
primary sequence to that of lipoprotein and hepatic lipase reveals conservation of the serine …