Ca2+ stores in insulin-secreting cells: lack of effect of cADP ribose
Ca 2+ stores were examined in several insulin secreting cell types by measuring uptake and
release of Ca 2+ by permeabilised cells. In pancreatic islet cells or INS-1 cells,< 20% of the
ATP-dependent, thapsigargin-sensitive Ca 2+ pool could be released by saturating
concentrations of inositol (1, 4, 5) P 3 (InsP 3). InsP 3 released> 60% of the thapsigargin-
sensitive Ca 2+ pool in RINm5F cells. The total Ca 2+ content of the thapsigargin-sensitive
pool was similar in each of these cell types. Neither cADP ribose (cADPR; 1 μM) nor caffeine …
release of Ca 2+ by permeabilised cells. In pancreatic islet cells or INS-1 cells,< 20% of the
ATP-dependent, thapsigargin-sensitive Ca 2+ pool could be released by saturating
concentrations of inositol (1, 4, 5) P 3 (InsP 3). InsP 3 released> 60% of the thapsigargin-
sensitive Ca 2+ pool in RINm5F cells. The total Ca 2+ content of the thapsigargin-sensitive
pool was similar in each of these cell types. Neither cADP ribose (cADPR; 1 μM) nor caffeine …