[HTML][HTML] Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function

T Ponomaryov, A Peled, I Petit… - The Journal of …, 2000 - Am Soc Clin Investig
T Ponomaryov, A Peled, I Petit, RS Taichman, L Habler, J Sandbank, F Arenzana-Seisdedos
The Journal of clinical investigation, 2000Am Soc Clin Investig
The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function.
Details of its regulation and sites of production are currently unknown. We report that in the
bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells.
Conditioning with DNA-damaging agents (ionizing irradiation, cyclophosphamide, and 5-
fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing
and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings …
The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function. Details of its regulation and sites of production are currently unknown. We report that in the bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells. Conditioning with DNA-damaging agents (ionizing irradiation, cyclophosphamide, and 5-fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings suggest that immature osteoblasts and endothelial cells control stem cell homing, retention, and repopulation by secreting SDF-1, which also participates in host defense responses to DNA damage.
The Journal of Clinical Investigation