A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity
T Decker, ML Lohmann-Matthes - Journal of immunological methods, 1988 - Elsevier
A simple way of measuring and evaluating lactate dehydrogenase release from lysed tumor
cells is described. LDH activity was determined as NADH oxidation or INT reduction over a
defined time interval, which was limited by stopping the enzymatic reaction with the inhibitor
oxamate. Reaction products were then assayed using a microplate reader. The principle of
measuring LDH activity of cellular culture supernatants as a measure of cytotoxicity was
successfully applied to a number of murine and human effector-target cell combinations …
cells is described. LDH activity was determined as NADH oxidation or INT reduction over a
defined time interval, which was limited by stopping the enzymatic reaction with the inhibitor
oxamate. Reaction products were then assayed using a microplate reader. The principle of
measuring LDH activity of cellular culture supernatants as a measure of cytotoxicity was
successfully applied to a number of murine and human effector-target cell combinations …