On the preparation of cryosections for immunocytochemistry
G Griffiths, A McDowall, R Back, J Dubochet - Journal of ultrastructure …, 1984 - Elsevier
The key preparation steps in the Tokuyasu thawed frozen section technique for
immunocytochemistry, namely freezing, sectioning, thawing, and drying, were studied. A
spherical tissue culture cell was used as a model system. The frozen hydrated section
technique indicated that glutaraldehyde-fixed, 2.1 M sucrose-infused pellets of cells were
routinely vitrified by immersion in liquid nitrogen but water was crystallized when lower
sucrose concentrations (0.6–1 M) were used. Quantitative mass measurements showed that …
immunocytochemistry, namely freezing, sectioning, thawing, and drying, were studied. A
spherical tissue culture cell was used as a model system. The frozen hydrated section
technique indicated that glutaraldehyde-fixed, 2.1 M sucrose-infused pellets of cells were
routinely vitrified by immersion in liquid nitrogen but water was crystallized when lower
sucrose concentrations (0.6–1 M) were used. Quantitative mass measurements showed that …