Maximum-entropy three-dimensional reconstruction with deconvolution of the contrast transfer function: a test application with adenovirus
U Skoglund, LG Öfverstedt, RM Burnett… - Journal of structural …, 1996 - Elsevier
We have developed an objective, quantitative, and general algorithm to improve the fidelity
of three-dimensional reconstructions made from electron micrographs while at the same
time filtering much of the noise present in the recorded data. The new technique is called
constrained maximum entropy tomography (COMET). The essence of the method is that it
will produce the most featureless reconstruction that fits the projection data within their
observational accuracy. In particular, the COMET procedure will minimise the detrimental …
of three-dimensional reconstructions made from electron micrographs while at the same
time filtering much of the noise present in the recorded data. The new technique is called
constrained maximum entropy tomography (COMET). The essence of the method is that it
will produce the most featureless reconstruction that fits the projection data within their
observational accuracy. In particular, the COMET procedure will minimise the detrimental …