Publisher Summary In situ hybridization histochemistry (ISHH) can be used to detect the
transcribed messenger RNA (mRNAs) coding for neuropeptides (or for enzymes or other
proteins) in cells that express the corresponding genes. Tissue sections are incubated with
labeled complementary DNA (cDNA) or complementary (c) RNA probes that form hybrids
with the cellular mRNA, and the location of labeled cells is then determined usually by
autoradiography for radioactively tagged probes. The probes can be designed to distinguish …