Publisher Summary All three mammalian Prx proteins (Prx I, II, and III) were shown to exhibit
peroxidase activity toward H 2 0 2 in the presence of the thioredoxin system (thioredoxin,
thioredoxin reductase, and NADPH) or nonphysiological hydrogen donor DTT. This chapter
describes two different assay procedures for the detection of Prx enzymes and purification
procedures of mammalian Prx I, II, and III. The first assay procedure is based on the fact that
glutamine synthetase is inactivated by oxygen radicals generated from the thiol oxidation …