We selected three peptides from the germ‐line sequence of the Vβ8.2 and Jβ2.3 gene segments of the murine T cell receptor for antigen (TCR) which contained putative K^d‐ and L^d‐restricted epitopes. Immunization of BALB/c (H‐2^d) mice with the Vβ8.2(67–90) 23‐mer peptide 1 as well as the 15‐mer Vβ8.2(95–108)‐peptide 2 efficiently primed specific CD8⁺ cytotoxic T lymphocyte (CTL) responses in vivo against natural TCR‐Vβ8.2 epitopes. Vβ8.2⁺ T cells were not deleted in TCR peptide‐immunized mice because the fractions of Vβ8.2⁺ CD4⁺ and Vβ8.2⁺ CD8⁺ T cells in spleen and lymph nodes were not altered. The proliferative response of Vβ8.2⁺ T cells to stimulation by monoclonal antibody F23.2 was selectively suppressed (by 60–80%) in peptide‐immunized BALB/c mice, indicating partial anergy of this T subset. Immunization of BALB/c mice with the Jβ2.3‐derived peptide 3 stimulated a CD8⁺ CTL response against a class I‐restricted epitope within this Jβ segment that was also generated during natural “endogenous” processing of this self antigen. These data confirm the predictive value of major histocompatibility complex class I allele‐specific motifs. The described experiments indicate that TCR peptide‐primed CD8⁺ CTL recognize class I‐restricted, natural Vβ/Jβ‐TCR epitopes. Such anti‐TCR CTL may, thus, operate in Vβ‐specific immunoregulation of the T cell system suppressing their functional reactivity without deleting them.