Dendritic cells from nonobese diabetic mice exhibit a defect in NF-κB regulation due to a hyperactive IκB kinase

DJ Weaver, B Poligone, T Bui… - The Journal of …, 2001 - journals.aai.org
DJ Weaver, B Poligone, T Bui, UM Abdel-Motal, AS Baldwin, R Tisch
The Journal of Immunology, 2001journals.aai.org
Insulin-dependent diabetes mellitus (IDDM) is characterized by the T cell-mediated
destruction of insulin-producing β cells. Accordingly, APCs, such as macrophage, have also
been shown to be important in the disease process. However, the role (s) of dendritic cells
(DCs) that exhibit potent APC function remains undefined in IDDM. Here we demonstrate
that DCs derived from nonobese diabetic (NOD) mice, a model for IDDM, are more sensitive
to various forms of stimulation compared with those from C57BL/6 and BALB/c mice …
Abstract
Insulin-dependent diabetes mellitus (IDDM) is characterized by the T cell-mediated destruction of insulin-producing β cells. Accordingly, APCs, such as macrophage, have also been shown to be important in the disease process. However, the role (s) of dendritic cells (DCs) that exhibit potent APC function remains undefined in IDDM. Here we demonstrate that DCs derived from nonobese diabetic (NOD) mice, a model for IDDM, are more sensitive to various forms of stimulation compared with those from C57BL/6 and BALB/c mice, resulting in increased IL-12 secretion. This property is a consequence of hyperactivation of NF-κB, a transcription factor known to regulate IL-12 gene expression. Specifically, NOD DCs exhibit persistent hyperactivation of both IκB kinase and NF-κB in response to stimuli, in addition to selective degradation of IκBε. Transfection of NOD DCs with a modified form of IκBα significantly reduced IL-12 secretion, suggesting that hyperactivation of NF-κB was in part responsible for increased IL-12 production. An enhanced capacity of NOD DCs to secrete IL-12 would be expected to contribute to the development of pathogenic Th1 (Tc1) cells during the diabetogenic response.
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