Suppression of the TPA-induced expression of nuclear-protooncogenes in mouse epidermis by crocetin via antioxidant activity.

JD Hsu, FP Chou, MJ Lee, HC Chiang, YL Lin… - Anticancer …, 1999 - europepmc.org
JD Hsu, FP Chou, MJ Lee, HC Chiang, YL Lin, SJ Shiow, CJ Wang
Anticancer research, 1999europepmc.org
Crocetin, a major component of the fruit of Gardenia jasminoides Ellis, was investigated for
its antitumor promoting effect on 12-O-tetradecanoylphorbol-13-acetate-promoted mouse
skin carcinogenesis. Topical application of 5 nmol TPA to CD-1 mice once daily for 5 days
caused epidermal hyperplasia, and increases in the levels of c-Fos, c-Jun and c-Myc in the
suprabasal layer of epidermis and the muscle layer of dermis. Immunocytolochemical
examination showed that pretreatment of 1 mumol crocetin repressed the TPA-induced …
Crocetin, a major component of the fruit of Gardenia jasminoides Ellis, was investigated for its antitumor promoting effect on 12-O-tetradecanoylphorbol-13-acetate-promoted mouse skin carcinogenesis. Topical application of 5 nmol TPA to CD-1 mice once daily for 5 days caused epidermal hyperplasia, and increases in the levels of c-Fos, c-Jun and c-Myc in the suprabasal layer of epidermis and the muscle layer of dermis. Immunocytolochemical examination showed that pretreatment of 1 mumol crocetin repressed the TPA-induced epidermal hyperplasia and the expressions of c-Jun, c-Fos and c-Myc to the extent of 47, 44 and 45% respectively. Crocetin of 3.0 mumol exhibited stronger inhibition on the induced hyperplasia and the oncoproteins levels (by 60, 53 and 55% respectively). Western blotting analysis confirmed this inhibitory effect of crocetin. Pretreatment of crocetin also repressed the TPA-induced H2O2 production and myeloperoxidase activity. These data indicate that crocetin suppresses the TPA-induced skin carcinogenesis maybe via its antioxidant property which, in turn, leads to a reduction in the TPA-induced expressions of c-Jun, c-Fos and c-Myc in mouse epidermis.
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