[HTML][HTML] Interferon-γ can stimulate post-proteasomal trimming of the N terminus of an antigenic peptide by inducing leucine aminopeptidase
J Beninga, KL Rock, AL Goldberg - Journal of Biological Chemistry, 1998 - ASBMB
Most antigenic peptides presented on major histocompatibility complex class I molecules
are generated during protein breakdown by proteasomes, whose specificity is altered by
interferon-γ (IFN-γ). When extended versions of the ovalbumin-derived epitope SIINFEKL
are expressed in vivo, the correct C terminus is generated by proteasomal cleavage, but
distinct cytosolic protease (s) generate its N terminus. To identify the other protease (s)
involved in antigen processing, we incubated soluble extracts of HeLa cells with the 11-mer …
are generated during protein breakdown by proteasomes, whose specificity is altered by
interferon-γ (IFN-γ). When extended versions of the ovalbumin-derived epitope SIINFEKL
are expressed in vivo, the correct C terminus is generated by proteasomal cleavage, but
distinct cytosolic protease (s) generate its N terminus. To identify the other protease (s)
involved in antigen processing, we incubated soluble extracts of HeLa cells with the 11-mer …