[HTML][HTML] Characterization of AMP-activated Protein Kinase β and γ Subunits: ASSEMBLY OF THE HETEROTRIMERIC COMPLEX IN VITRO (∗)
A Woods, PCF Cheung, FC Smith, MD Davison… - Journal of Biological …, 1996 - ASBMB
There is growing evidence that mammalian AMP-activated protein kinase (AMPK) plays a
role in protecting cells from stresses that cause ATP depletion by switching off ATP-
consuming biosynthetic pathways. The active form of AMPK from rat liver exists as a
heterotrimeric complex and we have previously shown that the catalytic subunit is
structurally and functionally related to the SNF1 protein kinase from Saccharomyces
cerevisiae. Here we describe the isolation and characterization of the two other …
role in protecting cells from stresses that cause ATP depletion by switching off ATP-
consuming biosynthetic pathways. The active form of AMPK from rat liver exists as a
heterotrimeric complex and we have previously shown that the catalytic subunit is
structurally and functionally related to the SNF1 protein kinase from Saccharomyces
cerevisiae. Here we describe the isolation and characterization of the two other …