Epithelial cell shedding in acute renal injury
LC Racusen - Clinical and experimental pharmacology and …, 1998 - Wiley Online Library
LC Racusen
Clinical and experimental pharmacology and physiology, 1998•Wiley Online Library1. To address the postulate that sublethally injured tubular cells may be shed from renal
epithelium while still viable, studies were undertaken in vivo in human 'acute tubular
necrosis' and in rabbit models of renal tubular injury. 2. Substantial numbers of viable
tubular cells were voided in the urine. When placed in culture, these cells gave rise to
monolayers, confirming viability. The majority of intact cells demonstrated markers of
proximal tubule. 3. In vitro studies of human renal proximal tubular cells exposed to …
epithelium while still viable, studies were undertaken in vivo in human 'acute tubular
necrosis' and in rabbit models of renal tubular injury. 2. Substantial numbers of viable
tubular cells were voided in the urine. When placed in culture, these cells gave rise to
monolayers, confirming viability. The majority of intact cells demonstrated markers of
proximal tubule. 3. In vitro studies of human renal proximal tubular cells exposed to …
1. To address the postulate that sublethally injured tubular cells may be shed from renal epithelium while still viable, studies were undertaken in vivo in human ‘acute tubular necrosis’ and in rabbit models of renal tubular injury.
2. Substantial numbers of viable tubular cells were voided in the urine. When placed in culture, these cells gave rise to monolayers, confirming viability. The majority of intact cells demonstrated markers of proximal tubule.
3. In vitro studies of human renal proximal tubular cells exposed to hypoxia/anoxia showed rounding and retraction associated with disruption of actin microfilaments. Phalloidin stabilized the filaments and prevented the changes in cell shape indicative of altered adherence.
Presented at the Experimental Biology Symposium on the Role of Integrins in Acute Renal Failure, New Orleans, Louisiana, USA, 1997.
Wiley Online Library